Experimental protein engineeringO. Michielin
Optimization and characterization of anti-cancer T-cell receptorsM. B. Irving, N. Rufer and O. Michielin
Based on computer modelling conducted at SIB of TCR-MHCp complexes of known structure, single and multiple amino acid changes are applied to TCRs and affinity and kinetic data are obtained for recombinant, soluble TCRs. Optimized TCRs are then displayed, via a lentivrial expression system, on the surface of human CD8 T cells and tested in vitro for their ability to target and destroy cancer cells. Ultimately it is hoped that such optimized TCRs can successfully be used for the adoptive immunotherapeutic treatment of cancer patients. This project is part of a highly collaborative effort between the Swiss Institute of Bioinformatics (SIB), the Multidisciplinary Center for Cancer Research (CePo), the Vaudois University Hospital Center (CHUV), the Swiss Institute for Experimental Cancer Research (ISREC) and the Ludwig Institute for Cancer Research (LICR).